2012年8月27日星期一

Laser pointer technical issues need to be addressed as soon as possible

Laser pointer technical issues need to be addressed as soon as possible.
Determination of membrane fluidity: laser scanning confocal microscope using a special computer software, membrane fluidity of quantitative and qualitative analysis of the membrane phospholipid acid composition analysis, drug action and site of action, reaction temperature measurement and comparison of species, and other plays important role. Time and play a role in the light-activated technology: laser scanning confocal microscope with light activation measurement functions, you can control the the instantaneous decomposition caged probe light wavelength and irradiation time, thereby artificially to control a variety of biologically active products, and other compounds space, this study can form a cage compounds, many of the key lock active material (such as neurotransmitters, intracellular second messenger camps, nucleotides, calcium, and some fluorescence, etc.) in cell proliferation, differentiation and other biological role in metabolic processes. Laser pointer technology research has achieved good results.
With this introduction, the powerful confocal microscope has been obvious. And other biological technologies (such as immunohistochemistry, in situ hybridization, etc.), to further expand the scope of testing. We almost qualitative and quantitative detection of intracellular biochemical composition of any one using a confocal microscope. Confocal laser scanning microscope (confocal laser scanning microscope) is a high-resolution micro-imaging technology. Quality laser pointer has been improved technical problems have been solved.
The ordinary fluorescence optical microscope thick samples (such as cells) was observed, the observation points neighborhood structure resolved fluorescence may interfere with the formation of larger. Confocal microscopy, the key point is that every space is only one point (focus) imaging, and then little by little to form a two-dimensional or three-dimensional image of the specimen through a computer-controlled scanning. In this process, the focus of the optical signal from the external image undisturbed, thus greatly improving the resolution capability of the clarity and detail of microscopic images. Removing the laser pointer not by the users themselves.
Figure 1 is a general operation principle of confocal microscopy FIG. The fluorescence excitation laser beam (laser) through the entrance aperture (light source pinhole () is a dichroic mirror dichroic mirror) reflection, be seen through the microscope objective lens (lens) together events after the sample (sample) internal focus (focus) In. Company produces laser fluorescence (fluorescent light), and a small amount of reflected laser light is collected again and sent to the destination of the dichroic mirror. The longer wavelength of the fluorescence image information are carried out directly through the dichroic mirror and through the outlet aperture (pinhole detection) to reach the detector (detectors) (usually a photomultiplier tube (PMT) or avalanche photodiode (diode)) , an electrical signal is sent to the computer. The laser pointer should be demolished professionals.

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